Inhibition by monensin of human cytomegalovirus DNA replication
Identifieur interne : 001579 ( Main/Exploration ); précédent : 001578; suivant : 001580Inhibition by monensin of human cytomegalovirus DNA replication
Auteurs : C. J. Kaiser [Allemagne] ; K. Radsak [Allemagne]Source :
- Archives of Virology [ 0304-8608 ] ; 1987-09-01.
English descriptors
- Teeft :
- Arch virol, Biol chem, Bovine serum albumin, Cell surface, Consecutive hcmv, Culture medium, Cytomegalovirus, Cytoplasmic, Cytoplasmic extracts, Drug addition, Drug treatment, Federal republic, Fibroblast, Glycoprotein, Glycoprotein synthesis, Hcmv, Hcmv hcmv hcmv hcmv, Herpes, Herpes simplex virus, Herpes simplex virus type, Human cytomegalovirus, Human cytomegmovirus, Human fibroblasts, Infectious cycle, Inhibitor, Inhibitory effect, Insoluble material, Intraeellular transport, Isopycnic centrifugation, Labelling, Marker proteins, Molecular weights, Monensin, Monensin treatment, Nitrocellulose sheets, Nuclear extracts, Other hand, Parallel cultures, Polymerase, Polymerase activity, Polypeptide, Posttranslational protein modification, Precursor, Precursor incorporation, Pulse interval, Pulse labelling, Replication, Simplex, Structural proteins, Tritiated sugars, Tritiated thymidine, Untreated, Various concentrations, Viral, Viral antigen, Viral replication, Virol, Virology.
Abstract
Summary: Monensin, at concentrations which depended on the multiplicity of infection, was found to prevent DNA replication of human cytomegalovirus (HCMV) as well as production of viral progeny in human foreskin fibroblasts. The drug did not affect DNA replication of herpes simplex virus. Inhibition of consecutive HCMV DNA synthesis was also observed following delayed addition of the drug within 12–24 hours postinfection, but was fully reversible upon its removal. Viral replication proceeded, however, without impairment in cultures treated with monensin prior to infection. Induction of viral DNA polymerase activity was not impeded by the inhibitor. Analysis of protein- and glycoprotein synthesis revealed that monensin interfered with the production of a number of HCMV-specific polypeptides. Furthermore, evidence was obtained that the drug may hinder intracellular transport of a 135 kd glycopolypeptide.
Url:
DOI: 10.1007/BF01310716
Affiliations:
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Le document en format XML
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J." last="Kaiser">C. J. Kaiser</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Zentrum für Hygiene und Medizinische Mikrobiologie, Institut für Virologie der Philipps-Universität, Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author><author><name sortKey="Radsak, K" sort="Radsak, K" uniqKey="Radsak K" first="K." last="Radsak">K. Radsak</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Zentrum für Hygiene und Medizinische Mikrobiologie, Institut für Virologie der Philipps-Universität, Marburg</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Giessen</region><settlement type="city">Marbourg</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1987-09-01">1987-09-01</date><biblScope unit="volume">94</biblScope><biblScope unit="issue">3-4</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="245">245</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Arch virol</term><term>Biol chem</term><term>Bovine serum albumin</term><term>Cell surface</term><term>Consecutive hcmv</term><term>Culture medium</term><term>Cytomegalovirus</term><term>Cytoplasmic</term><term>Cytoplasmic extracts</term><term>Drug addition</term><term>Drug treatment</term><term>Federal republic</term><term>Fibroblast</term><term>Glycoprotein</term><term>Glycoprotein synthesis</term><term>Hcmv</term><term>Hcmv hcmv hcmv hcmv</term><term>Herpes</term><term>Herpes simplex virus</term><term>Herpes simplex virus type</term><term>Human cytomegalovirus</term><term>Human cytomegmovirus</term><term>Human fibroblasts</term><term>Infectious cycle</term><term>Inhibitor</term><term>Inhibitory effect</term><term>Insoluble material</term><term>Intraeellular transport</term><term>Isopycnic centrifugation</term><term>Labelling</term><term>Marker proteins</term><term>Molecular weights</term><term>Monensin</term><term>Monensin treatment</term><term>Nitrocellulose sheets</term><term>Nuclear extracts</term><term>Other hand</term><term>Parallel cultures</term><term>Polymerase</term><term>Polymerase activity</term><term>Polypeptide</term><term>Posttranslational protein modification</term><term>Precursor</term><term>Precursor incorporation</term><term>Pulse interval</term><term>Pulse labelling</term><term>Replication</term><term>Simplex</term><term>Structural proteins</term><term>Tritiated sugars</term><term>Tritiated thymidine</term><term>Untreated</term><term>Various concentrations</term><term>Viral</term><term>Viral antigen</term><term>Viral replication</term><term>Virol</term><term>Virology</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Monensin, at concentrations which depended on the multiplicity of infection, was found to prevent DNA replication of human cytomegalovirus (HCMV) as well as production of viral progeny in human foreskin fibroblasts. The drug did not affect DNA replication of herpes simplex virus. Inhibition of consecutive HCMV DNA synthesis was also observed following delayed addition of the drug within 12–24 hours postinfection, but was fully reversible upon its removal. Viral replication proceeded, however, without impairment in cultures treated with monensin prior to infection. Induction of viral DNA polymerase activity was not impeded by the inhibitor. Analysis of protein- and glycoprotein synthesis revealed that monensin interfered with the production of a number of HCMV-specific polypeptides. Furthermore, evidence was obtained that the drug may hinder intracellular transport of a 135 kd glycopolypeptide.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Giessen</li><li>Hesse (Land)</li></region><settlement><li>Marbourg</li></settlement></list><tree><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Kaiser, C J" sort="Kaiser, C J" uniqKey="Kaiser C" first="C. J." last="Kaiser">C. J. Kaiser</name></region><name sortKey="Radsak, K" sort="Radsak, K" uniqKey="Radsak K" first="K." last="Radsak">K. Radsak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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